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sba clonotypingtm system hrp elisa kit  (SouthernBiotech)


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    SouthernBiotech sba clonotypingtm system hrp elisa kit
    Sba Clonotypingtm System Hrp Elisa Kit, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 95/100, based on 356 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sba+clonotyping+system+hrp/SBA+Clonotyping+System-HRP/pm41735326-347-5-10
    Average 95 stars, based on 356 article reviews
    sba clonotypingtm system hrp elisa kit - by Bioz Stars, 2026-09
    95/100 stars

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    Article Title: Development of an anti-SARS-CoV-2 monoclonal antibody panel and its applicability as a reagent in high-throughput fluorescence reduction neutralization and immunohistochemistry assays.
    Article Snippet: MAbs were concentrated through ammonium sulfate precipitation, and mAb isotypes were determined using SBA Clonotyping System‐HRP (Southern Biotech), according to the manufacturer's protocol.

    Article Title: A novel neutralizing monoclonal antibody recognizes a linear antigenic epitope of the spike protein of swine acute diarrhoea syndrome coronavirus.
    Article Snippet: The heavy and light chain subclasses were determined using the SBA Clonotyping System-HRP (Southern Biotech, Birmingham, AL, USA).

    Article Title: Generation and Application of Monoclonal Antibodies against Porcine S100A8, S100A9, and S100A12 Proteins Using Hybridoma Technology
    Article Snippet: The immunoglobulin subtype of the collected antibodies was determined through the SBA Clonotyping System-HRP (Southern-Biotech, Birmingham, AL, USA) according to the manufacturer’s instructions [ ].

    Article Title: Collagen type X alpha-1 assay
    Article Snippet: The isotype of antibody in supernatant was tested using the isotype determination kit, SBA Clonotyping System-HRP (5300-05, Southern Biotech).

    Article Title: Generation and Application of Monoclonal Antibodies against Porcine S100A8, S100A9, and S100A12 Proteins Using Hybridoma Technology.
    Article Snippet: The immunoglobulin subtype of the collected antibodies was determined through the SBA Clonotyping System-HRP (Southern-Biotech, Birmingham, AL, USA) according to the manufacturer’s instructions [39].

    Article Title: IFI16/IFI202 blockade suppresses tumor growth through CD8 + T-cell–mediated immunity
    Article Snippet: The antibody isotype was determined by the SBA Clonotyping System-HRP (SouthernBiotech) and Goat Anti-Mouse IgG2c, Human/Bovine/Horse (SouthernBiotech).

    Article Title: A novel neutralizing monoclonal antibody recognizes a linear antigenic epitope of the spike protein of swine acute diarrhoea syndrome coronavirus
    Article Snippet: The heavy and light chain subclasses were determined using the SBA Clonotyping System-HRP (Southern Biotech, Birmingham, AL, USA).

    Article Title: IFI16/IFI202 blockade suppresses tumor growth through CD8 + T-cell-mediated immunity.
    Article Snippet: The antibody isotype was determined by the SBA Clonotyping System-HRP (SouthernBiotech) and Goat Anti-Mouse IgG2c, Human/Bovine/Horse (SouthernBiotech).



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    a Reactivity of wtVLP and sVLP with anti-PV1 pAb or mAb234 in <t>ELISA.</t> b D antigen levels in the wtVLP or sVLP preparations. The data are means ± SD from three independent measurements. c Thermostability of PV1 sVLP. Equal amounts of PV1 sVLP were subjected to incubation at different temperatures for 10 min and then analyzed by ELISA using the pAb or mAb234. The red dashed line and dotted line indicate the mAb234 reactivity (OD450nm values) corresponding to 50% and 10% of the D -antigen in the untreated VLPs, respectively. Representative data from two independent experiments were shown.
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    a Reactivity of wtVLP and sVLP with anti-PV1 pAb or mAb234 in <t>ELISA.</t> b D antigen levels in the wtVLP or sVLP preparations. The data are means ± SD from three independent measurements. c Thermostability of PV1 sVLP. Equal amounts of PV1 sVLP were subjected to incubation at different temperatures for 10 min and then analyzed by ELISA using the pAb or mAb234. The red dashed line and dotted line indicate the mAb234 reactivity (OD450nm values) corresponding to 50% and 10% of the D -antigen in the untreated VLPs, respectively. Representative data from two independent experiments were shown.
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    a Reactivity of wtVLP and sVLP with anti-PV1 pAb or mAb234 in ELISA. b D antigen levels in the wtVLP or sVLP preparations. The data are means ± SD from three independent measurements. c Thermostability of PV1 sVLP. Equal amounts of PV1 sVLP were subjected to incubation at different temperatures for 10 min and then analyzed by ELISA using the pAb or mAb234. The red dashed line and dotted line indicate the mAb234 reactivity (OD450nm values) corresponding to 50% and 10% of the D -antigen in the untreated VLPs, respectively. Representative data from two independent experiments were shown.

    Journal: NPJ Vaccines

    Article Title: Structural insight into the assembly and D antigenicity of polio type 1 stabilized virus-like particles

    doi: 10.1038/s41541-026-01404-0

    Figure Lengend Snippet: a Reactivity of wtVLP and sVLP with anti-PV1 pAb or mAb234 in ELISA. b D antigen levels in the wtVLP or sVLP preparations. The data are means ± SD from three independent measurements. c Thermostability of PV1 sVLP. Equal amounts of PV1 sVLP were subjected to incubation at different temperatures for 10 min and then analyzed by ELISA using the pAb or mAb234. The red dashed line and dotted line indicate the mAb234 reactivity (OD450nm values) corresponding to 50% and 10% of the D -antigen in the untreated VLPs, respectively. Representative data from two independent experiments were shown.

    Article Snippet: Antibody isotypes were measured using the SBA Clonotyping TM System/HRP ELISA kit (Southern Biotech, USA) according to the manufacturer’s protocol.

    Techniques: Enzyme-linked Immunosorbent Assay, Incubation

    a Summary of mAb characteristics. b Reactivity of the mAbs to PV1 sVLP in ELISA. c Reactivity of the mAbs to PV1 wtVLP. d Side-by-side comparison of mAb234 and 3G10 in PV1 sVLP thermostability analysis. The purple dashed line and the dotted line indicate the mAb234 reactivity (OD450 nm values) corresponding to 50% and 10% of the unheated PV1 sVLP antigen. The red dashed line and the dotted line indicate the 3G10 reactivity (OD450 nm values) corresponding to 50% and 10% of the unheated PV1 sVLP antigen. e The binding kinetics of the mAb 3G10 to immobilized PV1 sVLP were measured by bio-layer interferometry (BLI). f , g Competitive ELISA. PV1 sVLP-coated wells were first incubated with different concentrations of the indicated mAbs, followed by detection with HRP-conjugated 3G10. The red dashed line and the dotted line indicate the HRP-conjugated 3G10 reactivity (OD450 nm values) corresponding to 100% and 50% of the PV1 sVLP antigen.

    Journal: NPJ Vaccines

    Article Title: Structural insight into the assembly and D antigenicity of polio type 1 stabilized virus-like particles

    doi: 10.1038/s41541-026-01404-0

    Figure Lengend Snippet: a Summary of mAb characteristics. b Reactivity of the mAbs to PV1 sVLP in ELISA. c Reactivity of the mAbs to PV1 wtVLP. d Side-by-side comparison of mAb234 and 3G10 in PV1 sVLP thermostability analysis. The purple dashed line and the dotted line indicate the mAb234 reactivity (OD450 nm values) corresponding to 50% and 10% of the unheated PV1 sVLP antigen. The red dashed line and the dotted line indicate the 3G10 reactivity (OD450 nm values) corresponding to 50% and 10% of the unheated PV1 sVLP antigen. e The binding kinetics of the mAb 3G10 to immobilized PV1 sVLP were measured by bio-layer interferometry (BLI). f , g Competitive ELISA. PV1 sVLP-coated wells were first incubated with different concentrations of the indicated mAbs, followed by detection with HRP-conjugated 3G10. The red dashed line and the dotted line indicate the HRP-conjugated 3G10 reactivity (OD450 nm values) corresponding to 100% and 50% of the PV1 sVLP antigen.

    Article Snippet: Antibody isotypes were measured using the SBA Clonotyping TM System/HRP ELISA kit (Southern Biotech, USA) according to the manufacturer’s protocol.

    Techniques: Enzyme-linked Immunosorbent Assay, Comparison, Binding Assay, Competitive ELISA, Incubation

    a Sequence alignment showing that the 3G10 epitope is identical among the wild-type Mahoney, the Mahoney-SC7, and the Sabin-1 strains. The key residues of the 3G10 epitope are indicated by red arrowheads. b Reactivities of serially diluted cIPV international standard detected by sandwich ELISAs with rabbit anti-PV1 pAb as the capture antibody and the indicated mAbs as the detection antibody. The X -axis shows the amounts of PV1 D -antigen in diluted cIPV samples and is in log10 scale. c Reactivities of serially diluted cIPV international standard detected by sandwich ELISAs with 5C6 as the capture antibody and HRP-conjugated 3G10 (3G10-HRP) as the detection antibody. The X -axis shows the amounts of PV1 D -antigen in diluted cIPV samples and is in log10 scale. d Standard curve generated by the 5C6/3G10-HRP-based sandwich ELISA with the international cIPV as reference. The raw data are the same as in ( c ) but were re-plotted with the X -axis in a linear scale.

    Journal: NPJ Vaccines

    Article Title: Structural insight into the assembly and D antigenicity of polio type 1 stabilized virus-like particles

    doi: 10.1038/s41541-026-01404-0

    Figure Lengend Snippet: a Sequence alignment showing that the 3G10 epitope is identical among the wild-type Mahoney, the Mahoney-SC7, and the Sabin-1 strains. The key residues of the 3G10 epitope are indicated by red arrowheads. b Reactivities of serially diluted cIPV international standard detected by sandwich ELISAs with rabbit anti-PV1 pAb as the capture antibody and the indicated mAbs as the detection antibody. The X -axis shows the amounts of PV1 D -antigen in diluted cIPV samples and is in log10 scale. c Reactivities of serially diluted cIPV international standard detected by sandwich ELISAs with 5C6 as the capture antibody and HRP-conjugated 3G10 (3G10-HRP) as the detection antibody. The X -axis shows the amounts of PV1 D -antigen in diluted cIPV samples and is in log10 scale. d Standard curve generated by the 5C6/3G10-HRP-based sandwich ELISA with the international cIPV as reference. The raw data are the same as in ( c ) but were re-plotted with the X -axis in a linear scale.

    Article Snippet: Antibody isotypes were measured using the SBA Clonotyping TM System/HRP ELISA kit (Southern Biotech, USA) according to the manufacturer’s protocol.

    Techniques: Sequencing, Generated, Sandwich ELISA